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BioGRID | Network Layout for KAR2 for Saccharomyces cerevisiae
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KAR2

 Saccharomyces cerevisiae
The BioGRID Database Seperator
Aliases: GRP78, YJL034W
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Description: ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p|Kar2p; protein coding
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Links: [Entrez-Gene]  [GeneDB]  [Germ Online]  [SGD]  
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Gene Ontology: 9 total GO mappings for this record. [view list]
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Download interactions associated with KAR2

KAR2 was identified with 48 protein interactions and 31 genetic interactions
Displaying:
 Name Aliases The BioGRID Database Seperator Description
Evidence Code(s)
Role Source(s)
The BioGRID Database Seperator
 SEC63
  • PTL1
  • YOR254C
    		•  The BioGRID Database Seperator Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER|Sec63p; protein coding
    Gene Ontology: [View List]
    Biochemical Activity
    bait Corsi AK (1997)
    bait McClellan AJ (1998)
    Reconstituted Complex
    bait Corsi AK (1997)
    bait McClellan AJ (1998)
    Synthetic Lethality
    hit Scidmore MA (1993)
    Synthetic Rescue
    bait Lyman SK (1995)
    bait Scidmore MA (1993)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SIL1
  • SLS1
  • YOL031C
    		•  The BioGRID Database Seperator Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein|Sil1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Steel GJ (2004)
    bait Kabani M (2002)
    bait Tyson JR (2000)
    Reconstituted Complex
    bait Kabani M (2000)
    Two-hybrid
    bait Kabani M (2000)
    Synthetic Lethality
    bait Kabani M (2000)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     IRE1
  • ERN1
  • YHR079C
    		•  The BioGRID Database Seperator Ire1p|Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait/hit Okamura K (2000)
    bait Todd-Corlett A (2007)
    bait Kimata Y (2007)
    Dosage Rescue
    bait Umebayashi K (1999)
    Phenotypic Enhancement
    bait Schuldiner M (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SCJ1
  • YMR214W
    		•  The BioGRID Database Seperator One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins|Scj1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    bait Gavin AC (2006)
    hit Collins SR (2007)
    Synthetic Lethality
    bait Schlenstedt G (1995)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     LHS1
  • CER1
  • SSI1
  • YKL073W
    		•  The BioGRID Database Seperator Lhs1p|Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; member of the Hsp70 family; localizes to the lumen of the ER; regulated by the unfolded protein response pathway; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Steel GJ (2004)
    Dosage Lethality
    hit Baxter BK (1996)
    Synthetic Lethality
    bait Hamilton TG (1996)
    bait Craven RA (1996)
    bait Baxter BK (1996)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YOS9
  • YDR057W
    		•  The BioGRID Database Seperator ER quality-control lectin; integral subunit of the HRD ligase; serves as a receptor for misfolded N-glycosylated proteins, participates in their targeting to ERAD; member of the OS-9 protein family|Yos9p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Denic V (2006)
    Affinity Capture-Western
    bait Denic V (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ROT1
  • YMR200W
    		•  The BioGRID Database Seperator Essential ER membrane protein; may be involved in protein folding; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations|Rot1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Takeuchi M (2006)
    Synthetic Lethality
    bait/hit Takeuchi M (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YNL313C
  • YNL313C
    		•  The BioGRID Database Seperator Putative protein of unknown function; identified as interacting with Kar2p, Grs1p, and Tub3p in high-throughput TAP-tagging experiments; YNL313C is an essential gene|Ynl313cp; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Hazbun TR (2003)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     GAS1
  • CWH52
  • GGP1
  • YMR307W
    		•  The BioGRID Database Seperator Beta-1,3-glucanosyltransferase, required for cell wall assembly; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor|Gas1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait/hit Fujita M (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     LYS9
  • LYS13
  • YNR050C
    		•  The BioGRID Database Seperator Lys9p|Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, which is the seventh step in lysine biosynthesis pathway; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEC23
  • YPR181C
    		•  The BioGRID Database Seperator GTPase-activating protein; component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat, involved in ER to Golgi transport and autophagy; stimulates the GDP-bound form of Sar1p|Sec23p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CYS4
  • NHS5
  • STR4
  • VMA41
  • YGR155W
    		•  The BioGRID Database Seperator Cys4p|Cystathionine beta-synthase, catalyzes the synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEC16
  • LPF1
  • YPL085W
    		•  The BioGRID Database Seperator COPII vesicle coat protein required for ER transport vesicle budding and autophagosome formation; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p|Sec16p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SKP1
  • MGO1
  • YDR328C
    		•  The BioGRID Database Seperator Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase|Skp1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait/hit Tagwerker C (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SMT3
  • YDR510W
    		•  The BioGRID Database Seperator Smt3p|Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait/hit Panse VG (2004)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     APOB
  • FLDB
  • EG338
    		•  The BioGRID Database Seperator apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
    Gene Ontology: [View List]
    Biochemical Activity
    hit Hrizo SL (2007)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ECM39
  • ALG12
  • YNR030W
    		•  The BioGRID Database Seperator Alg12p|Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation; protein coding
    Gene Ontology: [View List]
    PCA
    hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     RPT1
  • CIM5
  • YTA3
  • YKL145W
    		•  The BioGRID Database Seperator One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p|Rpt1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Ng W (2007)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     IDP1
  • YDL066W
    		•  The BioGRID Database Seperator Idp1p|Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Collins SR (2007)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEC61
  • YLR378C
    		•  The BioGRID Database Seperator Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER|Sec61p; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    bait Wittke S (2002)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEC31
  • WEB1
  • YDL195W
    		•  The BioGRID Database Seperator Essential phosphoprotein component (p150) of the COPII coat of secretory pathway vesicles, in complex with Sec13p; required for ER-derived transport vesicle formation|Sec31p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEC10
  • YLR166C
    		•  The BioGRID Database Seperator Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis|Sec10p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait/hit De Craene JO (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     PSA1
  • MPG1
  • SRB1
  • VIG9
  • YDL055C
    		•  The BioGRID Database Seperator GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure|Psa1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SOD1
  • CRS4
  • YJR104C
    		•  The BioGRID Database Seperator Cytosolic superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans|Sod1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     HTA2
  • H2A2
  • YBL003C
    		•  The BioGRID Database Seperator Hta2p|One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     HRD3
  • YLR207W
    		•  The BioGRID Database Seperator Hrd3p|Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events; protein coding
    Gene Ontology: [View List]
    Co-fractionation
    bait Plemper RK (1999)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     TDH2
  • GLD2
  • YJR009C
    		•  The BioGRID Database Seperator Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall|Tdh2p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     KAR2
  • GRP78
  • YJL034W
    		•  The BioGRID Database Seperator ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p|Kar2p; protein coding
    Gene Ontology: [View List]
    Co-purification
    hit Tokunaga M (1992)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SBA1
  • YKL117W
    		•  The BioGRID Database Seperator Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins and like p23 can regulate telomerase activity|Sba1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ERP2
  • YAL007C
    		•  The BioGRID Database Seperator Erp2p|Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SSH1
  • YBR283C
    		•  The BioGRID Database Seperator Ssh1p|Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    bait Wittke S (2002)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     TDH1
  • GLD3
  • YJL052W
    		•  The BioGRID Database Seperator Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall|Tdh1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VPS53
  • YJL029C
    		•  The BioGRID Database Seperator Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting|Vps53p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VPS52
  • SAC2
  • YDR484W
    		•  The BioGRID Database Seperator Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin|Vps52p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VPS54
  • CGP1
  • LUV1
  • TCS3
  • YDR027C
    		•  The BioGRID Database Seperator Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; potentially phosphorylated by Cdc28p|Vps54p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Gavin AC (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     STC2
  • TGL5
  • YOR081C
    		•  The BioGRID Database Seperator Tgl5p|Triacylglycerol lipase involved in TAG mobilization; localizes to lipid particles; potential Cdc28p substrate; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SPF1
  • COD1
  • PER9
  • PIO1
  • YEL031W
    		•  The BioGRID Database Seperator P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1|Spf1p; protein coding
    Gene Ontology: [View List]
    Phenotypic Enhancement
    hit Schuldiner M (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SPC2
  • SPY1
  • YML055W
    		•  The BioGRID Database Seperator Spc2p|Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25; protein coding
    Gene Ontology: [View List]
    Phenotypic Enhancement
    hit Schuldiner M (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     OST3
  • YOR085W
    		•  The BioGRID Database Seperator Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins|Ost3p; protein coding
    Gene Ontology: [View List]
    Phenotypic Enhancement
    hit Schuldiner M (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YJR015W
  • YJR015W
    		•  The BioGRID Database Seperator Putative