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BioGRID | Network Layout for NPL4 for Saccharomyces cerevisiae
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NPL4

 Saccharomyces cerevisiae
The BioGRID Database Seperator
Aliases: HRD4, YBR170C
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Description: Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation|Npl4p; protein coding
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Links: [Entrez-Gene]  [GeneDB]  [Germ Online]  [SGD]  
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Gene Ontology: 5 total GO mappings for this record. [view list]
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Download interactions associated with NPL4

NPL4 was identified with 41 protein interactions and 27 genetic interactions
Displaying:
 Name Aliases The BioGRID Database Seperator Description
Evidence Code(s)
Role Source(s)
The BioGRID Database Seperator
 CDC48
  • YDL126C
    		•  The BioGRID Database Seperator ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome|Cdc48p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait/hit Gavin AC (2002)
    bait Archambault V (2004)
    bait Krogan NJ (2006)
    bait/hit Gavin AC (2006)
    hit Collins SR (2007)
    Two-hybrid
    hit Ito T (2001)
    Affinity Capture-Western
    hit Rape M (2001)
    hit Hitchcock AL (2001)
    hit Gauss R (2006)
    bait/hit Schuberth C (2005)
    Reconstituted Complex
    bait/hit Rumpf S (2006)
    Synthetic Lethality
    hit Rape M (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     UFD1
  • YGR048W
    		•  The BioGRID Database Seperator Protein that interacts with Cdc48p and Npl4p, involved in recognition of polyubiquitinated proteins and their presentation to the 26S proteasome for degradation; involved in transporting proteins from the ER to the cytosol|Ufd1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait/hit Gavin AC (2002)
    bait Krogan NJ (2006)
    bait/hit Gavin AC (2006)
    hit Collins SR (2007)
    Two-hybrid
    hit Ito T (2001)
    hit Uetz P (2000)
    Affinity Capture-Western
    hit Rape M (2001)
    hit Hitchcock AL (2001)
    bait/hit Schuberth C (2005)
    Synthetic Lethality
    bait Rape M (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     UFD2
  • YDL190C
    		•  The BioGRID Database Seperator Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3|Ufd2p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Ho Y (2002)
    Co-purification
    bait Rumpf S (2006)
    Affinity Capture-Western
    bait Rumpf S (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SPT23
  • YKL020C
    		•  The BioGRID Database Seperator ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting|Spt23p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Shcherbik N (2007)
    Dosage Rescue
    hit Hitchcock AL (2001)
    Synthetic Rescue
    hit Hitchcock AL (2001)
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DOA1
  • UFD3
  • ZZZ4
  • YKL213C
    		•  The BioGRID Database Seperator Doa1p|WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein coding
    Gene Ontology: [View List]
    Co-purification
    bait Rumpf S (2006)
    Affinity Capture-Western
    bait Rumpf S (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     OTU1
  • YFL044C
    		•  The BioGRID Database Seperator Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family|Otu1p; protein coding
    Gene Ontology: [View List]
    Reconstituted Complex
    bait Rumpf S (2006)
    Affinity Capture-Western
    bait Rumpf S (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEL1
  • UBX2
  • YML013W
    		•  The BioGRID Database Seperator Protein involved in ER-associated protein degradation; proposed to coordinate the assembly of proteins involved in ERAD; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain|Ubx2p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait/hit Schuberth C (2005)
    Affinity Capture-MS
    bait Wilson JD (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     HRD1
  • DER3
  • YOL013C
    		•  The BioGRID Database Seperator Hrd1p|Ubiquitin-protein ligase required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Gauss R (2006)
    Synthetic Rescue
    hit Hitchcock AL (2003)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SSM4
  • DOA10
  • KIS3
  • YIL030C
    		•  The BioGRID Database Seperator Ssm4p|Ubiquitin-protein ligase of the ER/nuclear envelope, required for degradation of Alpha2p and other proteins containing a Deg1 degradation signal; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Carvalho P (2006)
    Synthetic Rescue
    hit Hitchcock AL (2003)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     MGA2
  • YIR033W
    		•  The BioGRID Database Seperator ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting|Mga2p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Shcherbik N (2007)
    Dosage Rescue
    hit Hitchcock AL (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ESA1
  • TAS1
  • YOR244W
    		•  The BioGRID Database Seperator Esa1p|Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression; protein coding
    Gene Ontology: [View List]
    Synthetic Lethality
    bait Lin YY (2008)
    Synthetic Growth Defect
    bait Lin YY (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CUE1
  • KIS4
  • YMR264W
    		•  The BioGRID Database Seperator Cue1p|Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Hitchcock AL (2003)
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     QRI8
  • UBC7
  • YMR022W
    		•  The BioGRID Database Seperator Ubc7p|Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Hitchcock AL (2003)
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YGL059W
  • YGL059W
    		•  The BioGRID Database Seperator Protein kinase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies|Ygl059wp; protein coding
    Gene Ontology: [View List]
    Biochemical Activity
    bait Ptacek J (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DER1
  • YBR201W
    		•  The BioGRID Database Seperator Der1p|Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Gauss R (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     KSP1
  • YHR082C
    		•  The BioGRID Database Seperator Ksp1p|Nonessential putative serine/threonine protein kinase of unknown cellular role; overproduction causes allele-specific suppression of the prp20-10 mutation; protein coding
    Gene Ontology: [View List]
    Biochemical Activity
    bait Ptacek J (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     PDC1
  • YLR044C
    		•  The BioGRID Database Seperator Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism|Pdc1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    hit Gavin AC (2002)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     HRD3
  • YLR207W
    		•  The BioGRID Database Seperator Hrd3p|Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Gauss R (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     NPL4
  • HRD4
  • YBR170C
    		•  The BioGRID Database Seperator Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation|Npl4p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait/hit Schuberth C (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YNL155W
  • YNL155W
    		•  The BioGRID Database Seperator Putative protein of unknown function, contains DHHC domain, also predicted to have thiol-disulfide oxidoreductase active site|Ynl155wp; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SBA1
  • YKL117W
    		•  The BioGRID Database Seperator Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins and like p23 can regulate telomerase activity|Sba1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     IFH1
  • YLR223C
    		•  The BioGRID Database Seperator Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate|Ifh1p; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     UBP3
  • YER151C
    		•  The BioGRID Database Seperator Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde transport between endoplasmic reticulum and Golgi compartments; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin|Ubp3p; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     PRP6
  • RNA6
  • TSM7269
  • YBR055C
    		•  The BioGRID Database Seperator Prp6p|Splicing factor, component of the U4/U6-U5 snRNP complex; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ARR4
  • GET3
  • YDL100C
    		•  The BioGRID Database Seperator ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress|Get3p; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DOA4
  • DOS1
  • MUT4
  • NPI2
  • YDR069C
    		•  The BioGRID Database Seperator Doa4p|Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     STO1
  • CBC1
  • CBP80
  • GCR3
  • YMR125W
    		•  The BioGRID Database Seperator Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80|Sto1p; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DFM1
  • YDR411C
    		•  The BioGRID Database Seperator Dfm1p|ER localized derlin-like family member involved in ER stress and homeostasis; not involved in ERAD or substrate retrotranslocation; interacts with CDC48; contains four transmembrane domains and two SHP boxes; protein coding
    Gene Ontology: [View List]
    Dosage Growth Defect
    hit Sato BK ()
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     OLE1
  • MDM2
  • YGL055W
    		•  The BioGRID Database Seperator Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria|Ole1p; protein coding
    Gene Ontology: [View List]
    Dosage Rescue
    hit Hitchcock AL (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     NOP1
  • LOT3
  • YDL014W
    		•  The BioGRID Database Seperator Nop1p|Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin; protein coding
    Gene Ontology: [View List]
    Synthetic Lethality
    bait Davierwala AP (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     RPN4
  • SON1
  • UFD5
  • YDL020C
    		•  The BioGRID Database Seperator Rpn4p|Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; protein coding
    Gene Ontology: [View List]
    Dosage Rescue
    hit Hitchcock AL (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     MOT2
  • NOT4
  • SIG1
  • YER068W
    		•  The BioGRID Database Seperator Component of the CCR4-NOT complex, which has multiple roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; with Ubc4p, ubiquitylates subunits of the nascent polypeptide-associated complex (NAC)|Mot2p; protein coding
    Gene Ontology: [View List]
    Synthetic Lethality
    bait Mulder KW (2007)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VPS27
  • DID7
  • GRD11
  • SSV17
  • YNR006W
    		•  The BioGRID Database Seperator Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)|Vps27p; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SRN2
  • SRN10
  • VPS37
  • YLR119W
    		•  The BioGRID Database Seperator Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus|Srn2p; protein coding
    Gene Ontology: [View List]
    Synthetic Rescue
    hit Auld KL (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
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